Table of Contents

1. Introduction to Dengue IgG/IgM Test
2. Mechanism of Dengue IgG/IgM Test
3. Dengue IgG/IgM Test Procedure
4. Interpretation of Test Results
5. QL Biotech Company Solutions
6. References

Introduction to Dengue IgG/IgM Test

The Dengue IgG/IgM test is a serological assay used to detect antibodies produced by the immune system in response to dengue virus infection. Dengue fever, caused by the dengue virus, is a mosquito-borne illness prevalent in tropical and subtropical regions worldwide. The test primarily helps in differentiating between primary and secondary infections, which is crucial for patient management.

Mechanism of Dengue IgG/IgM Test

The Dengue IgG/IgM test is based on the principle of enzyme-linked immunosorbent assay (ELISA). This test detects the presence of IgM and IgG antibodies in the patient's serum. IgM antibodies indicate recent infection, typically appearing within 4-10 days after the onset of symptoms, while IgG antibodies suggest a past infection or secondary dengue infection, typically rising 7-10 days after the onset.

Dengue IgG/IgM Test Procedure

The procedure for conducting a Dengue IgG/IgM test involves several key steps:

1. Sample Collection: A blood sample is collected from the patient and serum is separated.
2. Antigen Coating: Microtiter plates are coated with dengue virus antigens.
3. Serum Incubation: The patient's serum is added to the wells, allowing antibodies, if present, to bind to the coated antigens.
4. Detection: Conjugated secondary antibodies are added which bind to human IgG or IgM. A substrate is then introduced, leading to a color change measurable at a specific wavelength (typically 450nm).

Interpretation of Test Results

The results of the Dengue IgG/IgM test are interpreted as follows:

1. IgM Positive, IgG Negative: Suggests a primary dengue infection. Early detection is crucial to prevent complications.
2. IgM Positive, IgG Positive: Indicates a current secondary dengue infection due to a previous exposure to the virus.
3. IgM Negative, IgG Positive: Implies a past dengue infection and potential immunity.
4. IgM Negative, IgG Negative: Indicates no current or past infection.

QL Biotech Company Solutions

QL Biotech is at the forefront of developing advanced diagnostic solutions for infectious diseases, including dengue fever. Their Dengue IgG/IgM testing kits are known for high sensitivity and specificity rates, consistently above 95%. QL Biotech implements cutting-edge enzyme immunoassay technology to ensure reliable and rapid testing.

References

1. World Health Organization (WHO). Dengue Guidelines for Diagnosis, Treatment, Prevention, and Control.
2. Guzman, M.G., & Harris, E. (2015). Dengue. The Lancet, 385(9966), 453-465.
3. QL Biotech Official Website. Available from: [link to the website].